Genetic Studies of Induced Mutants in Melilotus alba . II. Inheritance and Complementation of Chlorophyll‐deficient Mutants 1

Genetic Studies of Induced Mutants in Melilotus alba. II. Inheritance and Complementation of Chlorophyll-deficient Mutants

Six ethyl methanesulfonate-induced mutants of Melilotus alba Desr. were studied. Five of the mutants behaved as monogenic recessives. In the sixth mutant, two independent recessive alleles were responsible for the observed phenotype. Of these two genes, one had a phenotypic effect similar to the five single-gene mutants. The other (the veined gene) caused a chlorophyll deficiency in which the l...

Mutants of Sweetclover (Melilotus alba) Lacking Chlorophyll b: Studies on Pigment-Protein Complexes and Thylakoid Protein Phosphorylation.

Mutants of sweetclover (Melilotus alba) with defects in the nuclear ch5 locus were examined. Using thin-layer chromatography and absorption spectroscopy, three of these mutants were found to lack chlorophyll (Chl) b. One of these three mutants, U374, possessed thylakoid membranes lacking the three Chl b-containing pigment-protein complexes (AB-1, AB-2, and AB-3) while still containing A-1 and A...

Genetic studies of acridine-induced mutants in Streptococcus pneumoniae.

The mutagenic properties of acridines on pneumococcus are described. All seven acridines tested were mutagenic at the amiA locus conferring a resistance to 10(-5) M aminopterin. The effects of quinacrine were more specifically investigated. It was observed that: mutants can be obtained only by treatment of exponentially growing cells; a sharp maximum mutagenic effect occurs at a concentration s...

Genetic Blocks in the Synthesis of Coumarin in Melilotus alba

Genetic studies of ad-8 mutants in Neurospora crassa. II. Interallelic complementation at the ad-8 locus.

N earlier studies with Neurospora, it was believed that with biochemical I mutants the heterocaryon test was a satisfactory test for allelism, based on the expectation that only heterocaryons between nonallelic mutants should be capable of growth on nonsupplemented media (BEADLE and COONRADT 1944). However, experiments by MITCHELL and MITCHELL (1956) suggested that a positive heterocaryon test ...